ABE 468 Microbiological Engineering
|
Week # |
Topic |
Book Reading |
|
1 |
Overview of MicrobiologyCell construction DNA, RNA and protein synthesis |
Ch. 2 Ch. 5 |
|
2 |
Bioenergetics Major metabolic pathways Strain development |
Ch. 5 Ch. 5 Ch. 8 |
|
3 |
Enzyme kinetics Competitive, noncompetitive, uncompetitive, and substrate inhibition Immobilized Enzyme Systems Design project proposal presentations |
Ch. 3
|
|
4 |
Stirred Tank Reactors Sterilization, medium and inoculum preparation Batch fermentation and microbial growth Fermentation Lab |
Ch. 6 |
|
5 |
Continuous fermentation and kinetics Optimization Project Report 1 on microbial and medium characteristics |
Ch. 6 |
|
6 |
Operating considerations Two- vessel fermentors First midterm exam |
Ch. 9 |
|
7 |
Fed-Batch Systems Recycled fermentation Immobilized Cell Systems |
Ch. 9 |
|
8 |
Aeration and oxygen transfer Project Report 2 on bioreactor design |
|
|
9 |
Spring Break |
Ch. 10 |
|
10 |
Agitation Scale-up strategies Aeration Lab |
Ch. 10 |
|
11 |
Product recovery and purification Separation of insoluble products; Filtration, centrifugation, coagulation & flocculation Project Report 3 on aeration and agitation |
Ch. 11 |
|
12 |
Cell disruption; mechanical and non-mechanical methods |
Ch. 11 |
|
13 |
Second midterm exam Separation of soluble products; Liquid-liquid extraction, precipitation, adsorption, dialysis, |
Ch. 11 |
|
14 |
reverse osmosis, ultra filtration, cross flow ultra- filtration Chromatography, electrophoresis, electrodialysis Project Report 4 on separation |
Ch. 11 |
|
15 |
Finishing steps of purification; Crystallization, drying Field Trip: Merck, Danville, PA |
Ch. 11 |
|
16 |
Final Design project reports and presentations |
|
|
17 |
Final Exam Week |
|
Required Text:
Michael L. Shuler and Fikret Kargi. 2002. Bioprocess Engineering : Basic Concepts. 2nd Edition. Prentice Hall, Englewood Cliffs, N.J.
Instructor: Grading:
Dr. Ali Demirci Homework 15%
231 Agricultural Eng. Bldg. Midterm Exams 30%
Phone: 863-1098 Project reports/presentation 40%
e-Mail: demirci@psu.edu Final exam 15%
Academic integrity, as defined by University Faculty Senate Policy 49-20, is the pursuit of scholarly activity free from fraud and deception and is an educational objective of this institution. Academic dishonesty includes, but is not limited to, cheating, plagiarizing, fabricating of information or citations, facilitating acts of academic dishonesty by others, having unauthorized possession of examinations, submitting work of another person or work previously used without informing the instructor, or tampering with the academic work of other students.
